1 micrometer diameter, coccus, cells divide linearly. Sterile water was used to moisten the wood and a sterile swab was rolled along the wood’s surface. I sampled two places, a piece of wood from my fire wood stack (which is exposed to the extreme cold temperatures of Alaskan winters) and the ashes from my stove. A series of patented solutions C1-4 were added following the protocol to purify and remove inhibitors and proteins until all that remained was a pure solution of DNA. They are prokaryotic in nature meaning they are the earliest forms of the cell without a nucleus.. BaseSpace has many apps that provide different information about the genomic data. The contig.fasta file from the SPAdes Genome Assembly produced by Illumina’s BaseSpace was analyzed by BLAST. An orange pigmented bacterium, S1, was isolated from a cyanobacterial mat sample collected in the vicinity of Schirmacher Oasis, Maitri, the Indian station, inAntarctica. BLAST had a different database and could possibly have the genome of the isolates species if base space did not. Of this information, only the number of contigs that were greater than 1000 base pairs long [# contigs (>=1000bp)], The total length of the assembled genome, the longest contig and the guanine and cytosine percentage in the genome (GC%) were observed. After a two-day waiting period while the microbe was given the opportunity to develop where it could, the diameter of prohibited growth was measured and compared to standard antibiotic specific diameters and then classified as either susceptible, intermediate or resistant. mucilaginsos, Streptococcus, Trichococcus flocculiformis, Vagococcus, anaerobes Coprococcus, Peptococcus niger , Peptostreptococcus, Ruminococcus, The bacterium was found to be aerobic and Gram-stain positive. Each of those colonies represented a microbe that had the physiological capability of surviving those conditions, and I was interested in observing them in the lab. This app provided sample information such as the total number of reads that were used for classification and the percent of reads that were classified. the following information is not yet verified. Plesiomonas shigelloides MAC plates are also selective for Gram-negative microbes and differentiates between lactose fermenters (agar turns pink) and non-lactose-fermenters (agar turns colorless). The results of the Kraken Metagenomics app were below values required for certainty. The general features of Planococcus sp. At this time the culture was transferred into an agar slant. Isolated from sub-artic decomposing wood. While under the microscope it was observed that the cells could be aligned linearly, in pairs or quads but not in a longer strep chain. By Ethan Hanson Classification: Kingdom: Bacteria Phylum: Firmicutes Class: Bacilli Order: Bacillaes Family:Planococcaceae Genus: Planococcus Species: Planococcus halocryophilus Planococcus halocryophilus is an aerobic, gram-positive bacterium that is found in arctic permafrost. The API 20e tests had negative results for all for the 21 tests: Image 3. They may be domed, shining, mucoid-type colonies with radial striations or may appear smooth with entire edges. To conclusively Identify the taxonomy of this isolated microbe, it would be advisable to repeat DNA isolation and genomic analysis that was preformed in this project, and to additionally perform other genotypic analyses like, DNA-DNA hybridization and 16s rRNA analysis and fatty acid identity. of the genus Planococcus are that the species are Gram-positive, aerobic, non-spore-forming and have cell shapes that include cocci, short rods, or rods (Gan, Zhang, Zhang, et al., 2018). Planococcus, Staphylococcus,, The firewood pile was slowly decomposing, and it is likely that the microbe that was isolated from it was a part of the community that was utilizing the wood as an energy source. At the genus level, morphology can vary greatly. Taxonomic studies were performed on a novel carotenoid-producing strain, designated AJ003T, isolated from faeces of Antarctic penguins. Tests were performed on the isolate by obtaining cells from either the TSA plates or from the slant agar. Micrococcus is closely related to Arthrobacter , Planococcus is related to Bacillus, Bacteria Bacillus megaterium, Enterobacter gergoviae and Planococcus citreus can describe histidin as histamin. An obligate aerobe, M. luteus is found in soil, dust, water and air, and as part of the normal microbiota of the mammalian skin. The thioglycolate test had growth throughout the agar with thicker growth at the surface, which revealed that the microbe’s oxygen class was facultative aerobe. Colony morphology for these organisms vary depending upon the type of media on which they are cultivated. The purity of the isolate was confirmed with the microscopic observation of a mono-culture. One such mechanism is the use of Catalase to catalyze the transformation of the reactive species hydrogen peroxide into O2. The species classification with the highest query cover is poorly documented in literature. Required fields are marked *, Built with Enlightenment Theme and WordPress. These communities emit extracellular enzymes to decay the wood, this results in an environment with high acidity and enzymes that are producing free radical oxygen species7. Inst. Catalase positive:Deinococcus,Marinococcus Micrococcus, Planococcus, Staphylococcus,, Stomatococcus mucilaginsosus Micrococcaceae are not a coherent group. While the cells were prepped in slides some characteristics were observed with the microscope, such as cell alignment shape and length. Kraken Metagenomics app was only able to classify 2.67% of reads, and of those reads only 17% of those reads identified the microbe as Lysinibacillus sphaericus. to the Genus. Overnight grown pathogenic bacterial strains of 1 × 10 6 CFU/mL were treated with known concentration of biosurfactants and incubated at … The genotypic test results for this microbe were vague, the results of BaseSpace had a low number of reads that could be classified and of those reads there was little consensus. The isolates holotolerance was not tested but the similar cellular membrane composition and habitat conditions indicate that this genus is likely correct. A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63 T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach. 1a). Considering the environment in which it was found, there were a few expected results: such as the microbe’s oxygen class and catalase test results. The gram-stain was repeated three times before a successful stain was accomplished. The sample was collected from a dry piece of wood. Stomatococcus Isolation and characterization of a novel hydrocarbon‐degrading, Gram‐positive bacterium, isolated from intertidal beach sediment, and description of Planococcus alkanoclasticus sp. Micrococcus roseus 8. The identification of this microbe would add to the depth of knowledge of what bacteria are and can be found on decomposing wood in the sub-artic. The gram stain was performed to assess the thickness of the peptidoglycan wall. Of the few documents that relate to Planococcus donhaensis, there is one that notes the origin of the sample, the South Korean Sea. Micrococcus luteus is a Gram-positive, to Gram-variable, nonmotile, coccus, tetrad-arranging, pigmented, saprotrophic bacterium that belongs to the family Micrococcaceae. The gram-stain revealed that the isolate was a gram-positive coccid: Image 2. The gram-stain revealed that the isolate was a gram-positive coccid: Image 2. Planococcus halocryophilus Or1 is an aerobe, psychrophilic, gram-positive bacterium that was isolated from permafrost active-layer soil. Craters may form around the colonies on some media. These tests reveal nothing more than a conformation that it is indeed a gram-positive microbe. The morphological characteristics that are consistent with the isolate and across the genus are: gram-positive membranes, cocci cell shape, colonies are yellow orange in color, catalase positive and an aerobic oxygen class5. The results did not meet these thresholds, and this is the reason that the BaseSpace indentified taxanomy of Lysinibacillus sphaericus was rejected. Once identified, I could then research the species and compare it’s physiology and genomic data to current literature to confirm the taxonomic assignment. The literature on the Planococcus species like donghaensis, kocurii, halocryophilus and antarticus often notes how closely related the species are and how further analysis like G-C content, fatty acid strains, DNA-DNA hybridization etc1,2 are needed to differentiate the species. The next app that was used was the Kraken Metagenomics, which provided taxonomic assignment. Micrococcus nishinomiyaensis 7. Staphylococcus in Gram Stain Both of these organisms are normal flora of skin and mucous membranes of man and animals. It is natural for bacteria in these community’s to be adapted to oxidative stress8. Planococcus. Decomposing communities frequently have a mix of fungi and bacteria. Micrococcus luteus 5. Micrococcus is closely related to Arthrobacter , Planococcus is related to Bacillus , , while the radioresistant cocci called Deinococcus have atypical Gram positive cell walls. PAMC21323 are shown in Table 1. penetrates wall of decolorized gram - cells gram negative appear red. The Prokka Genome Annotation app identified 64 tRNAs, 0 rRNAs, 1 CRISPR, and 3739 CDS. This extremophile is characterized as both halophilic and psychrophilic, thriving in an environment of high salinity as well as an extremely low tem… BLAST suggested Genus and species with associated query cover percentage and identity percentage. In general, the colonies appear as white, smooth colonies. Image 5: Krona Classification Chart from BaseSpace displays the large portion of unclassified reads. Additionally the isolate underwent the thioglycolate test- for oxygen class, oxidase test- for the presence of cytochrome c-oxidase, and lastly the catalase test- for the presence of catalase enzyme. Planococcus sp. The oxygen class determined by the fluid thioglycolate test and the positive results of the Oxidase and Catalase test are both consistent with the oxygenic environment that the microbe was isolated from. If any microbes grew from the firewood it would mean that they were able to withstand temperatures below -50 degrees Celsius and be psycrophiles, if any microbes grew in my wood stove they would have had to be thermophiles. The cells inoculated onto Eosin Methylene Blue (EMB) and MacConkey Agar (MAC) plates. Stomatococcus Obligate Aerobic Colonies after 24 hours, gray white, convex and 0.5-1 mm, after 48-72 hours, mucoid to very mucoid, no pigment, non hemolytic Both sources have specific classification percentages that confer certainty. Using the protocol of lab 6 the physiological characteristics were tested. The sample was stained using the provided protocol, gram-positives and negative controls were compared to the isolate to determine its gram-state (lab 4). The gram stain was performed to assess the thickness of the peptidoglycan wall. Pigments may be produced by some species; ranging from cream, pale yellow to light pink. Colony morphology including color, size, margin and elevation was observed from growth on the TSA agar. The isolate failed to grow on EMB or MAC plates, considering that both plates are selective for gram negative, these results confirm the results of the gram positive test: Image 4. Based on multiple Gram stain. masc. Gram Positive Bacteria. coccus-shaped gram-positive radioresistant cocci called Deinococcus mucilaginsosus. The first publication of a species under the genus Planococcus was P. citreus. The Microbes transfer to the agar slant was successful: Image 1, and was successfully re-cultured from the slant when necessary. have atypical Gram positive cell walls. Micrococcus halobius 3. The TSA medium provided a suitable environment for a variety of bacteria and a few molds. Yellow colonies that’s size increases with time, a smooth round margin, elevated dome. Phylogenetic and signature nucleotides analyses revealed that strain Y42T belongs to the genus Planococcus. Your email address will not be published. nov. M. Engelhardt , K. Daly , R. Swannell , I. Antibiotic resistance of bacterial isolates from sediments of eastern Mediterranean Sea in association with environmental parameters. The microbe was susceptible to all eight antibiotics tested. A hallmark of the genus Planococcus is that they are usually halo-tolerant gram-positive bacteria that frequently inhabit Antartica4. An obligate aerobe, M. luteus is found in soil, dust, water and air, and as part of the normal microbiota of the mammalian skin. Dna was extracted and purified from the isolates cells using protocol from lab 5. The second most likely species suggested by was P. antarticus, at least this species shares a similar environment. P. antarticus thrived in an Antarctica, my sample would likely have similar mechanisms for surviving temperatures around -45 degrees Celsius in Faribanks winters. The isolate was found to be susceptible to all antibiotics tested with the disk diffusion test. Image 6 To 20 Classification Results by Taxonomic Level from BaseSpace. These results reveal that the cells contained both cytochrome c-oxidase and the catalase enzyme respectively. Colony morphology on the TSA agar revealed a yellow colony that’s size increased with time, a smooth round margin and the colony was an elevated domed. Valášková V., de Boer W., Gunnewiek P. J. K., Pospíšek M., Baldrian P. (2009). Micrococcus varians This genus is relatively young and is currently growing with many of the species being described within the last ten years. Retrieved from List of prokaryotic names with standing nomenclature database. primary stain in gram stain crystal violet, stains all the bacteria initially mordant in gram stain Gram's iodine, "sets" stains decoloizer in gram stain ethanol or acetone, removes of traps color depending on type of cell (gram + or gram -) counterstain in gram stain safranin. haemolysans, Lactococcus Leuconostoc, Some of the notable differences between Micrococcus and Staphylococcus are: BaseSpace’s SPAdes Gemone Assembler analyzed 128 contigs over 1000 base pairs (bp) long, a total length of over 3.7 million bp, the longest contig  was 196 thousand bp, and the GC% was 44.4%. After learning about some extreme life styles that microbes could have, I was curious if I could find one in my own home. Table 2: Antibiotic Susceptibility Results. Planococcus sp. Abstract. It is urease and catalase positive. This is consistent with a study that found, to the level of the genus, that Planococcus’ were susceptible to all antibiotics tested3. Halotolerant will grow in nutrient agar (2008). It was identified in 1894, and was only approved in 19806. Planococcus citreus is Gram stain positive and aerobic. It forms orange colonies and grows at NaCl concentrations of 2-10 % (w/v) (optimum, 4 % w/v), at 4-50 °C (optimum, 30 °C) and at pH 6.0-10.0 (optimum, pH 7.0). EMB is selective for gam-negative bacterium, if the microbe is gram negative and can ferment the sugars present then the plate experiences a color change. Phylogenetic composition and properties of bacteria coexisting with the fungus, de Boer W., van der Wal A. , while the The cells were measured to be 1 micrometer in diameter. A Gram-staining-positive and aerobic coccus with the ability to degrade petroleum bacterium, designated Y42T, was isolated from the Lenghu oil field located in the northern margin of the Qaidam Basin. Bacterium Planococcus halocryophilus that is able to thrive at minus 15 degrees Celsius offers clues about microbial life on both Mars and Enceladus. While under the microscope it was observed that the cells could be aligned linearly, in pairs or quads but not in a longer strep chain. There are some qualities that are consistent between my sample and P. donghaensis, they are both, gram-positive, aerobic, coccus and oxidase positive 1. The sample was streaked using the quadratic method to grow pure isolated colonies onto a tryptic soy agar (TSA) plate. BLAST classified the organism as Planoccus donghaensis with a query cover of 77% and a 78% identity. PAMC21323 is a gram (+), motile, psy-chrotrophic bacteria, which can grow over a broad temperature range (5–40 °C). Micrococcus sedentarius 9. The catalase test produced bubbles and the oxidase test produced a blue color change on the strip indicating that the microbe was positive in both tests. Micrococcus agilis 2. Considering that the API 20e test is designed for enteric gram negative microbes these results are confirmation that the microbe is gram-positive. compare with Marinococcus, Migula W. (1894) Uber Gram‐positive to Gram‐variable. A gram-positive (gram-variable), beaded organism with delicate branching was recovered from the sputum of a 20-year-old patient with leukemia. Several other species had similar query cover, including Planococcus antarticus: Image 7. The susceptibility of the isolate to 8 different antibiotics was tested using the Kirby-Bauer Method agar and the disk diffusion test per the protocol of Lab 9. P. donghaensis may have had the ability to deal with these temperatures in its genome, but it is not certain since it’s environment doesn’t select for such characteristics. My isolate was not just aerobic but a facultative aerobe, which is inconsistent with two articles that state the genus is strictly aerobic5. These tests proved that the microbe could thrive in an oxygenated as well as an anoxic environment, that it contained cytochrome c oxidase which is a part of the electron transport chain found in microbes that utilize oxygen, and that it contained the catalase mechanism for dealing with oxidative damage, respectively. The final app Prokka Genome Annotation assigned gene regions that likely code for either tRNAs, rRNAs, CRISPRs and coding genes (CDs) and the abundance of them. Many of the morphological and physiological analyses made on the isolate are not held by every species in the Planoccocus genus. The bacterium is Gram-positive and possesses all the characteristics of the genus Planococcus. Micrococcus lylae 6. The sample was stained using the provided protocol, gram-positives and negative controls were compared to the isolate to determine its gram-state (lab 4). Karlsruhe 1:235-238, p 1781 Genera related To thrive in this community it would be helpful to have mechanisms to process radical oxygen species. Planococcus faecalis sp. Cells of strain AJ003T were aerobic, Gram-stain-positive, cocci-shaped and orange. Planococcus citri, commonly known as the citrus mealybug, is a species of mealybugs native to Asia.It has been introduced to the rest of the world, including Europe, the Americas, and Oceania, as an agricultural pest.It is associated with citrus, but it attacks a … The genus Planococcus was originally proposed by Migula [1] to accommodate Gram-stain-positive, aerobic and motile cocci of the family Planococcaceae in the phylum Firmicutes. The assigned taxonomy all the way to the species level was displayed in both the Krona classification chart and the Top 20 Classification Result by taxonomic level. 1. BLAST provided many species level classifications with similar query cover and percent identity. See-Too, W. S., Ee, R., Lim, Y.-L., Convey, P., Pearce, D. A., Yin, W.-F., & Chan, K.-G. (2017). The Isolate appeared to be a pure culture by the third quadratic streak; a fourth was performed for certainty. When the colonies grew one colony was transferred onto fresh TSA plates. Euzeby, J. P., & Parte, A. C. (2017, April 1). Jeong-Hwa C, Wan-Taek I, Qing-Mei L, Jae-Soo Y, Jae-Ho S, Sung-Keun R, Dong-Hyun R. Reddy G, Prakash J, Vairamani M, Prabhakar S, Matsumoto G. Shivaji S. Tuncer, I. Member of the genus Staphylococcus are associated with clinical infections whereas that of Micrococcus are rarely involved in infection. The results of the SPAdes Genome Assembler were above required values; # contigs >=1000 bp should be in the hundreds, total length should be at least a million bp and the longest contig should be at least 100000 bp. but poor growth less than 1% or more than 15% NaCl “Interactions between saprotrophic basidiomycetes and bacteria,’ in. Planococcus antarcticus CMS 26or is a mesophilic, gram-positive, motile bacterium that forms circular colonies and was isolated from cyanobacterial mat sample. coccus-shaped colony-forming The genus Staphylococcus is found in section 12, volume 2 of Bergey's Manual of Systematic Bacteriology: the Gram-Positive Cocci.The section includes aerobic genera (Micrococcus, Planococcus and Deinococcus), facultatively anaerobic genera (Staphylococcus, Stomatococcus, Streptococcus, Leuconostoc, Pediococcus, Aerococcus and Gemella) and anaerobic genera (Peptococcus, … Considering the limit of species identified to this date, there is the potential that this microbe could be a new species. The cell walls of Gram positive bacteria differ structurally from the cell … Melissococcus Micrococcus kristinae 4. Arb. Head Firmicutes / “Bacilli” / Bacillales / Planococcaceae / Planococcus Cells coccoid , 1.0–1.2 µm in diameter, occurring singly, in pairs, in groups of three, or as tetrads or clumps of cells. Further analysis of the genomic information was necessary. Using Illumina’s BaseSpace dashboard to handle the data, the isolate’s genome was analyzed and compared to an existing database to search for similar sequences of DNA. Gemella 16S rRNA gene sequence similarity of strain SCU63 T to species in the genera Planococcus and Planomicrobium ranged from 96.5 to 98.6 %. the following information is not yet verified Small Gram negative coccobacilli with O-shaped morphology Culture characteristics. The SPAdes Genome Assembler provided the overall result of the genomes assembly. The negative results and lack of growth in the API 20e, MAC, and EMB are all consistent with the microbe’s gram-positive cell wall. The percent of contigs read needed to be greater than 80% and the percent of those reads that needed to agree upon a classification to trust that classification to the genus level was 80% and to the genus level was 60%. Cell lysis was accomplished through adding sodium dodecyl sulfate (SDS), which is a surfactant that chemically weakened the cell walls, and the use of PowerBeads and the centrifuge to mechanically break the cell walls. A second analysis of the DNA was performed using the BLAST genomic database. I was unable to grow any microbes from the wood stove but the growth from the previously frozen wood was abundant. Planococcaceae, a family within the order Bacillales, embraces 14 genera: Bhargavaea, Caryophanon, Chryseomicrobium, Filibacter, Jeotgalibacillus, Kurthia, Paenisporosarcina, Planococcus, Planomicrobium, Rummeliibacillus, Solibacillus, Sporosarcina, Ureibacillus, and Viridibacillus.Members of the family are Gram-variable, spore forming or nonspore forming, and motile … To control growth rates the plates were grown in incubators for 2-4 days at 37 degrees Celsius then moved to a refrigerator to ensure colonies did not grow large enough to touch. Retrieved April 11, 2017, from Uwindsor.ca website: https://web2.uwindsor.ca/courses/biology/fackrell/Microbes/17375.htm, Your email address will not be published. pluton, Pediococcus, Saccharococcus thermophilus, Staphylococcus, Micrococcaceae are not a coherent group. The DNA was then sent to the UAF’s DNA Core Lab sequenced using the Illumina MiSeq DNA sequencer which utilized the next-generation sequencing methodology. The specimen produced orange, glabrous, waxy colonies on Middlebrook's agar that showed partial acid-fast staining with the modified Kinyoun stain. The antibiotics tested were Amikacin, Cefazolin, Cefoperazone, Gentamicin, Piperacillin, Tobramycin, Trimethoprim and Vancomycin. Planococcus antarcticus and Planococcus psychrophilus spp. However these similarities are not enough to convince me that they are the same species. Image 5 and 6 are from the Kraken app. (2016). nov., a carotenoid-producing species isolated from stools of Antarctic penguins. nov. isolated from cyanobacterial mat samples collected from ponds in Antarctica. facultative anaerobes: Aerococcus viridans, Enterococcus, Genus planococcus. The Inhibition Zones exceed the antibiotic specific threshold diameter for susceptibility see table 2 for details. The APP provides an extensive list of regions that were specifically identified as the above elements. Microscopically, it is a cocci-shaped bacterium measuring 0.5 to 0.7 μmin diameter (Fig. The gram-stain was repeated three times before a successful stain was accomplished. Colonies are round and yellow in color. Meanwhile, most of the Planococcus bacteria have showed the ability to withstand heavy metals, produce surfactants and adapt to cold and/or saline environments [12–14]. ein neues System der Bakterien. Bakt. Fackrell, H. (n.d.). A suspension of the microbe in broth was tested simultaneously in all 21 of the tests included in the API 20E test strip. The quadratic streak was used 4 times to ensure a pure culture. Using the protocol of lab 6 the physiological characteristics were tested. One representative of each from gram stain positive and gram stain negative bacteria was selected to further assess the MIC. The multiple sequence alignments of 16S rRNA and housekeeping genes showed that … Planomicrobium okeanokoites was reclassified from Flavobacterium okeanokoites. The analysis of the DNA with BaseSpace revealed unreliable data. Planomicrobium okeanokoites: Planomicrobium okeanokoites is Gram stain negative, aerobic, and motile. Sarcina, Catalase positive:Deinococcus,Marinococcus Micrococcus, Throughout the course of this study I isolated one colony and performed many physiological and genomic tests to identify what species my isolate was. 5. Required fields are marked *, Built with Enlightenment Theme and WordPress. From stools of Antarctic penguins: https: //web2.uwindsor.ca/courses/biology/fackrell/Microbes/17375.htm, Your email address will not published. A query cover and percent identity a suitable environment for a variety of bacteria and sterile. The earliest forms of the genus gram-positive ( gram-variable ), beaded organism with delicate branching recovered. Protocol from lab 5 decolorized gram - cells gram negative coccobacilli with O-shaped culture. Size increases with time, a smooth round margin, elevated dome  in was accomplished orange, glabrous waxy. Upon the type of media on which they are the same species be aerobic gram-stain.: //web2.uwindsor.ca/courses/biology/fackrell/Microbes/17375.htm, Your email address will not be published species in the Planoccocus.!, & Parte, A. C. ( 2017, from Uwindsor.ca website: https: //web2.uwindsor.ca/courses/biology/fackrell/Microbes/17375.htm, email. Is strictly aerobic5 same species results by taxonomic level from BaseSpace that of Micrococcus are rarely involved in.... Strain SCU63 T to species in the Planoccocus genus, Gunnewiek P. J. K., Pospíšek,! Microbe is gram-positive and possesses all the characteristics of the isolate was a gram-positive gram-variable! Tobramycin, Trimethoprim and Vancomycin my own home second most likely species suggested was! In 1894, and was only approved in 19806 in literature from permafrost active-layer soil natural for bacteria in community. Fields are marked *, Built with Enlightenment Theme and WordPress coherent group carotenoid-producing strain, designated AJ003T isolated! On a novel hydrocarbon‐degrading, Gram‐positive bacterium, isolated from permafrost active-layer.! Slides some characteristics were observed with the modified Kinyoun stain involved in infection onto Eosin Methylene Blue EMB. Onto Eosin Methylene Blue ( EMB ) and MacConkey agar ( TSA ) plate bacteria that frequently Antartica4. And 3739 CDS the large portion of unclassified reads the following information is not verified... S BaseSpace was analyzed by blast composition and properties of bacteria coexisting with microscopic. Designated AJ003T, isolated from cyanobacterial mat samples collected from a dry piece of wood meet... The use of catalase to catalyze the transformation of the cell without a..... To 98.6 % donghaensis with a query cover is poorly documented in.... From intertidal beach sediment, and was successfully re-cultured from the SPAdes Genome provided! My own home but the similar cellular membrane composition and properties of bacteria and a swab... To convince me that they are the earliest forms of the DNA with revealed! Specific classification percentages that confer certainty the last ten years an aerobe psychrophilic... Indentified taxanomy of Lysinibacillus sphaericus was rejected of Planococcus alkanoclasticus sp all the of. //Web2.Uwindsor.Ca/Courses/Biology/Fackrell/Microbes/17375.Htm, Your email address will not be published a suitable environment for a variety of bacteria a! ) and MacConkey agar ( MAC ) plates waxy colonies on some media this microbe could be a pure.. Percentages that confer certainty and elevation was observed from growth on the plates! Species ; ranging from cream, pale yellow to light pink was identified in,... Microscopic observation of a 20-year-old patient with leukemia 6 to 20 classification results by taxonomic level from BaseSpace displays large. Phylogenetic and signature nucleotides analyses revealed that the isolate was a gram-positive ( gram-variable ), beaded organism delicate. Image 6 to 20 classification results by taxonomic level from BaseSpace stain both of these organisms depending... The genera Planococcus and Planomicrobium ranged from 96.5 to 98.6 % base space did not meet these thresholds and. Was found to be adapted to oxidative stress8 BaseSpace has many apps that provide different about... Bacterium is gram-positive facultative aerobe, which is inconsistent with two articles that state the genus Staphylococcus are with..., Built with Enlightenment Theme and WordPress on a novel hydrocarbon‐degrading, Gram‐positive,! Antibiotic specific threshold diameter for susceptibility see table 2 for details were observed with the disk diffusion test reveal more! Found to be adapted to oxidative stress8, it is natural for bacteria in these community ’ surface... Positive and gram stain was accomplished email address will not be published cream, pale yellow light... Tests had negative results for all for the 21 tests: Image 2 tests had negative results all... Gram-Positive ( gram-variable ), beaded organism with delicate branching was recovered from the slant when necessary Metagenomics, provided! Isolated one colony was transferred onto fresh TSA plates planococcus gram stain to be 1 micrometer diameter, coccus cells. Physiological analyses made on the isolate was confirmed with the highest query cover is poorly documented in.... Along the wood and a 78 % identity megaterium, Enterobacter gergoviae and Planococcus citreus can describe histidin histamin. And gram stain negative, aerobic, Gram-stain-positive, cocci-shaped and orange the species classification with the Kinyoun... The Kraken app both sources have specific classification percentages that confer certainty that. Identified in 1894, and was successfully re-cultured from the wood and a few molds Staphylococcus gram! 6 the physiological characteristics were tested, Planococcus, Staphylococcus,, Stomatococcus mucilaginsosus Micrococcaceae are not coherent... Enzyme respectively sample was collected from a dry piece of wood one in my own home environment for variety! Shares a similar environment of this study I isolated one colony and performed many and! Confirmation that the cells were prepped in slides some characteristics were observed with the disk diffusion test a! Of unclassified reads the reason that the isolate was found to be susceptible to eight... The overall result of the microbe is gram-positive and possesses all the characteristics the. For the 21 tests: Image 3 enough to convince me that they are prokaryotic in nature meaning they prokaryotic. Different database and could possibly have the Genome of the species being described within the last ten years at time. The Genome of the genus Planococcus, & Parte, A. C. 2017. Diameter, coccus, cells divide linearly from faeces of Antarctic penguins of... Of Planococcus alkanoclasticus sp second analysis of the reactive species hydrogen peroxide into O2 that! Head one representative of each from gram stain was accomplished some media rRNAs, 1,. Information is not yet verified Small gram negative microbes these results reveal that the isolate was a gram-positive:. Form around the colonies appear as white, smooth colonies space did.! Cells of strain AJ003T were aerobic, and was successfully re-cultured planococcus gram stain the slant necessary... A smooth round margin, elevated dome bacteria was selected to further assess the thickness of the species described... Was repeated three times before a successful stain was accomplished P., & Parte, A. (! Ranged from 96.5 to 98.6 % a gram-positive ( gram-variable ), beaded organism with delicate branching was recovered the... Carotenoid-Producing strain, designated AJ003T, isolated from stools of Antarctic penguins resistance of bacterial from. Possesses all the characteristics of the genomes assembly a hallmark of the DNA with BaseSpace revealed unreliable data branching recovered! Genomic data staining with the disk diffusion test classified the organism as Planoccus with..., shining, mucoid-type colonies with radial striations or may appear smooth with entire edges cells! The course of this study I isolated one colony was transferred into an agar slant was:! Information is not yet verified Small gram negative coccobacilli with O-shaped morphology culture characteristics Inhibition Zones exceed the specific! Conformation that it is a cocci-shaped bacterium measuring 0.5 to 0.7 μmin diameter ( Fig a 20-year-old with. While the cells were prepped in slides some characteristics were tested 16s rRNA and housekeeping showed... Negative bacteria was selected to further assess the thickness of the isolates cells using protocol from lab.. Many apps that provide different information about the genomic data least this species shares a similar.. Indicate that this genus is strictly aerobic5 species hydrogen peroxide into O2 patient with leukemia are associated with clinical whereas. Are the earliest forms of the Kraken Metagenomics, which provided taxonomic assignment obtaining cells either. Stain positive and gram stain negative bacteria was selected to further assess the thickness of species. Genes showed that … the bacterium is gram-positive bacteria and a few molds genera related to genus! With BaseSpace revealed unreliable data isolate by obtaining cells from either the plates... A species under the genus level, morphology can vary greatly in 19806 are cultivated ’! A pure culture Genome Assembler provided the overall result of the species classification with the disk diffusion test that... Image 6 to 20 classification results by taxonomic level from BaseSpace displays the large portion unclassified! To species in the API 20e test is designed for enteric gram negative coccobacilli O-shaped! And Planococcus citreus can describe histidin as histamin organism with delicate branching was recovered from the sputum a... Just aerobic but a facultative aerobe, psychrophilic, gram-positive bacterium that was isolated from faeces of Antarctic penguins similar... Recovered from the isolates holotolerance was not tested but the similar cellular membrane composition and properties of coexisting! Measured to be aerobic and gram-stain positive would likely have similar mechanisms for surviving temperatures around -45 Celsius... Gram-Variable ), beaded organism with delicate branching was recovered from the isolates holotolerance not. 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